PFLink

Basic instructions

Use the Centroid tab to upload your centroid data. Use the Envelope tab in addition to centroid data if you have isotopic envelope data (only with HDExaminer at this time).

If you are using the 'Custom' data format or HDX Workbench, please upload it to the centroid data section, regardless of whether you have isotopic envelopes in the file.

Each file upload only accepts one file. The online tool only processes one state/protein at a time within that file.

If you have multiple state/protein(s) in that file, you must select the one you want to process.

You can download example files using the 'Download example data' button below.

The flags-file and custom-format CSV can be found inside the example data download zip.

If the option does not say optional, then it is required.

Example datasets

Global settings

Flags file (.txt) - optional. A flags file can be used to autofill the global settings. It is not necessary, but it can speed up entering data.

File settings - choose your HDX input file format.

Protein rename and more - optional. Renames your state/protein in your output HXMS file. As well as forcing a replicate ID or bimodal group

Peptide inclusion/exclusion (.csv) - optional. Filters peptides that you want to include/exclude in your output HXMS file.

Submit and output data

HXMS file uploads